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Disruption of the LINC complex impairs endothelial barrier. To completely disrupt the LINC complex, we utilized a previously characterized dominant negative peptide, known as DN-KASH, in which overexpression of this peptide saturates available binding sites on SUN proteins and displaces endogenous nesprin isoforms from the nuclear envelope (Lombardi et al., 2011). mCherry-tagged dominant ...

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Abstract. Our understanding of the principles underlying the protein-folding problem can be tested by developing and characterizing simple models that make predictions which can be compared to experimental data. Here we extend our earlier model of folding free energy landscapes, in which each residue is considered to be either folded as in the ...

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